mentzer index sensitivity, specificity

A total of 136 children were confirmed to have IDA with serum ferritin levels ≤ 12 ng/dL. Distinguishing β-TT from IDA has important clinical implications because each disease has an entirely different cause, prognosis, and treatment. Mentzer index, England and Fraser index, RDW index and Ehsani index each, showed highest specificity (99.66% each). The Ehsani et al. Mentzer Index sensitivity (Sn) was 0.36 with specificity (Sp) at 0.81, positive predictive value (PPV) at 0.44 and negative predictive value (NPV) at 0.75. RBC count has been considered a valuable index [15], but we showed that RBC count had only 70.5% specificity and 65.3% Youden’s index. RDW index has highest positive predictive value (90.91%), while Green and King index has highest negative predictive value (98.87%). To find out more, see our, Browse more than 100 science journal titles, Read the very best research published in IOP journals, Read open access proceedings from science conferences worldwide, Published under licence by IOP Publishing Ltd, IOP Conference Series: Earth and Environmental Science. None of the subjects had a combined case of IDA and β-TT. The samples were obtained during the course of routine analysis and collected in EDTA anticoagulant tubes. [2]. D. A. Rathod, A. Kaur, V. Patel et al., “Usefulness of cell counter-based parameters and formulas in detection of, E. Urrechaga, L. Borque, and J. F. Escanero, “The role of automated measurement of RBC subpopulations in differential diagnosis of microcytic anemia and, C. Thomas and L. Thomas, “Biochemical markers and hematologic indices in the diagnosis of functional iron deficiency,”, W. C. Mentzer Jr., “Differentiation of iron deficiency from thalassaemia trait,”, I. … Patients with β-TT and concomitant iron deficiency may show normal HbA2 levels. Therefore, the RBC count is normal with a low MCV and the Mentzer's index is <13. RBC count was higher in the β-TT (5.56 ± 0.4) group than that in the IDA (4.84 ± 0.59; ). Mentzer index OR value was 2.4 (0.5 - 11.5, CI95%). It may be that interpopulation differences in the effectiveness of various RBC indices for discriminating β-TT from IDA could be attributed to differences in the mutation spectrum of the thalassemia disease in different populations [22]. The RDWI values were increased in both groups: 17.4 ± 3.48 in the IDA group and 16.76 ± 1.83 in patients with β-TT (). The authors declare that there is no conflict of interests regarding the publication of this paper. This process helps to select appropriate individuals for a more detailed examination; however, no study has found 100% specificity or sensitivity for any of these RBC indices. They have a sensitivity of 73.5% and 81.8% and specificity of 94.8% and 95.7% respectively. Trivedi Dhara et al. The IDA group consisted of children with Hb levels of 8.7–11.4 g/dL, mean corpuscular volume (MCV) < 80 fL at age > 6 years or MCV < 70 fL at age < 6 years, serum iron level < 30 μg/dL, transferrin saturation < 16%, and serum ferritin level ≤ 12 ng/dL. index > Shine and Lal index > MCHD. A high erythrocyte count (RBC > 5.0 × 106/μL) is a common feature of IDA and β-TT. Keywords: Mentzer Index, diagnostic value, thalassemia 1. Data were taken from 37 anemia patients at Prof. Dr. Margono Soekarjo Regional Public Hospital in Purwokerto who underwent Hb electrophoresis examination using electrophoresis instrument and routine blood count. index [9], and the Sirdah et al. The purpose of using indices to discriminate anemia is to detect subjects who have a high probability of requiring appropriate follow-up and to reduce unnecessary investigative costs. Additionally, Table 4 shows that the highest and lowest Youden’s index values belonged to the Mentzer index (81%) and MCHD (5.8%). Thalassemia diagnosis requires gen examination using Polymerase Chain Reaction (PCR) device or hemoglobin (Hb) electrophoresis using electrophoresis or High Performance Liquid Chromatography (HPLC) device which is only available in certain hospitals. Methods. Methods. The diagnosis of β-TT involves measuring the HbA2 concentration of lysed RBCs via HPLC. According to our data, the Mentzer index had the highest Youden’s index for correctly distinguishing β-TT and IDA at 81%. Mentzer index could be used to predict the diagnosis of beta-thalassemia carrier. Mentzer Index sensitivity (Sn) was 0.36 with specificity (Sp) at 0.81, positive predictive value (PPV) at 0.44 and negative predictive value (NPV) at 0.75. SI and total iron binding capacity (TIBC) were determined calorimetrically (Siemens Advia 2400 Chemistry Analyzer), and ferritin was measured by immunoassay using a Siemens Advia XP Analyzer. It is also important to note that the test has a high sensitivity and low specificity. They found that the Mentzer index had 85% sensitivity, 93% specificity, and 79% Youden’s index [20]. None of the subjects of the present study had a combined case of β-TT and IDA. Anemia resulting from lack of sufficient iron to synthesize hemoglobin is the most common hematological disease in infants and children. Aysel Vehapoglu, Gamze Ozgurhan, Ayşegul Dogan Demir, Selcuk Uzuner, Mustafa Atilla Nursoy, Serdar Turkmen, Arzu Kacan, "Hematological Indices for Differential Diagnosis of Beta Thalassemia Trait and Iron Deficiency Anemia", Anemia, vol. None of the indices was completely sensitive or specific in distinguishing β-TT and IDA. Red cell values at various ages of study groups are shown in Table 5. Volume 255, According to our results, the percentage of correctly diagnosed patients was the highest with the Mentzer index (91%) followed by the Ehsani et al. We are committed to sharing findings related to COVID-19 as quickly as possible. Sensitivity and specificity of Mentzer index for the detection of β-TT were 100% and 69.4%, respectively. We retrospectively evaluated the reliability of various indices for differential diagnosis of microcytosis and β-TT in the same patient groups. demonstrated that RDWI had the highest sensitivity (78.9%), that the England and Fraser index had the highest specificity and highest Youden’s index (99.1 and 64.2%, resp. Shine and S. Lal, “A strategy to detect, J. M. England and P. M. Fraser, “Differentiation of iron deficiency from thalassaemia trait by routine blood-count,”, P. C. Srivastava, “Differentiation of thalassemia minor from iron deficiency,”, R. Green and R. King, “A new red cell discriminant incorporating volume dispersion for differentiating iron deficiency anemia from thalassemia minor,”, B. M. Ricerca, S. Storti, G. d'Onofrio et al., “Differentiation of iron deficiency from thalassaemia trait: a new approach,”, M. Sirdah, I. Tarazi, E. Al Najjar, and R. Al Haddad, “Evaluation of the diagnostic reliability of different RBC indices and formulas in the differentiation of the, M. A. Ehsani, E. Shahgholi, M. S. Rahiminejad, F. Seighali, and A. Rashidi, “A new index for discrimination between iron deficiency anemia and beta-thalassemia minor: results in 284 patients,”, O. A. Telmissani, S. Khalil, and T. R. George, “Mean density of hemoglobin per liter of blood: a new hematologic parameter with an inherent discriminant function,”, A. Mosca, R. Paleari, G. Ivaldi, R. Galanello, and P. C. Giordano, “The role of haemoglobin A(2) testing in the diagnosis of thalassaemias and related haemoglobinopathies,”, E. J. Harthoorn-Lasthuizen, J. Lindemans, and M. M. A. C. Langenhuijsen, “Influence of iron deficiency anaemia on haemoglobin A(2) levels: possible consequences for, A. Demir, N. Yaralı, T. Fısgın, F. Duru, and A. Kara, “Most reliable indices in differentiation between thalassemia trait and iron deficiency anemia,”, D. Aslan and Ç. Altay, “Incidence of high erythrocyte count in infants and young children with iron deficiency anemia: re-evaluation of an old parameter,”, M. Ferrara, L. Capozzi, R. Russo, F. Bertocco, and D. Ferrara, “Reliability of red blood cell indices and formulas to discriminate between, S. M. AlFadhli, A. M. Al-Awadhi, and D. AlKhaldi, “Validity assessment of nine discriminant functions used for the differentiation between Iron deficiency anemia and thalassemia minor,”, M. Ghafouri, L. Mostaan Sefat, and L. Sharifi, “Comparison of cell counter indices in differention of beta thalassemia trait and iron deficiency anemia,”, F. Rahim and B. Keikhaei, “Better differential diagnosis of iron deficiency anemia from beta-thalassemia trait,”, G. Ntaios, A. Chatzinikolaou, Z. Saouli et al., “Discrimination indices as screening tests for, C. Rosatelli, G. B. Leoni, T. Tuveri et al., “Heterozygous, I. El-Agouza, A. Abu Shahla, and M. Sirdah, “The effect of iron deficiency anaemia on the levels of haemoglobin subtypes: possible consequences for clinical diagnosis,”, P. R. Dallman, “Blood and blood-forming tissue,” in. S&L had 87.63% specificity and 93.4% NPV. Kerman index showed a sensitivity and specificity of 84.25% and 77.79%, for IDA and 77.79% and 84.25% beta thalassemia respectively. Electronic cell counters have been used to determine red cell indices as a first indicator of β-TT. Youden index which tells about the overall performance of a diagnostic test was highest for Kerman 1 index (65.4%). Batebi et al reported sensitivity and specificity of Mentzer index as 86.3% and 85.4% respectively. index > RBC count > Sirdah et al. The two other categories of microcytic anemia are Iron Deficiency Anemia and Anemia of Chronic Disease. In this study, King-Green formula was reported to have the highest sensitivity and specificity, which as a result, was the most reliable formula followed by Mentzer formula. Mentzer index OR value was 2.4 (0.5 - 11.5, CI95%). When the Mentzer index was calculated, 264 children with microcytic anemia (91%) were correctly diagnosed. BibTeX and the Shine and Lal indices demonstrated the highest sensitivity (100%) but had low specificities for correctly identifying IDA (14.7%) and β-TT (10.2%). compared nine discriminant functions in patients with microcytic anemia and measured validity using Youden’s index. We calculated 12 discrimination indices in all patients with hemoglobin (Hb) values of 8.7–11.4 g/dL. Citation W Siswandari et al 2019 IOP Conf. The Mentzer index showed good sensitivity, specificity, and Youden’s index values of 98.7%, 82.3%, and 81%, respectively. Statistical analysis of data was done by SPSS 16 software. We retrospectively analyzed 290 children with microcytic anemia (mean age: 6.2 ± 4.2 years; range: 1.1–16 years). This study aims at figuring out the diagnostic value of Mentzer Index in predicting beta-thalassemia carrier compared to the results of HB electrophoresis examination. We observed that the RBC count increased at the initiation of iron therapy in patients with IDA and decreased by the end of therapy. Mentzer Index sensitivity (Sn) was 0.36 with specificity (Sp) at 0.81, positive predictive value (PPV) at 0.44 and negative predictive value (NPV) at 0.75. 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Rdwi showed the highest accuracy, sensitivity, 90.9 % ; specificity, %... Committee of Faculty of Medicine, Bezmialem Vakif University, Turkey different ethnic populations, β-TT is the common! And concomitant iron deficiency anemia ( mean age: 6.2 ± 4.2 years ;:... Iron deficiency anemia ( 91 % of children with microcytic anemia are beta carriers... Specificity was as Kerman > Mentzer > Serivastava during the course of routine analysis and collected EDTA! Early stages of the indices was completely sensitive or specific in distinguishing β-TT from has. Children aged 1.1–16 years ) were evaluated Green and King index > MDHL > Ricerca et.! K and RDWI showed the highest reliabilities for differentiating β-TT from IDA using a... Indices was completely sensitive or specific in distinguishing β-TT from IDA using a! Attribution 3.0 licence now, many investigators have used different mathematical indices distinguish... 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Initiation of iron therapy 70.5 %, and HbA2 values were determined all. Had a combined case of IDA and β-TT in the β-TT ( 5.56 ± )... And 92 % NPV group than that in the same patient groups trait ( β-TT ) and iron anemia... Ratio of SI to TIBC value order of the Local Ethics Committee of Faculty Medicine! Concomitant iron deficiency anemia ( IDA ) and Fraser index had the lowest sensitivity of 66.2 %,.! Misdiagnosis of β-TT has consequences for potential homozygous offspring RDWI > Srivastava index RDWI... Rbc count increased at the initiation of iron therapy correctly diagnosed is highest for Kerman 1 (. Are committed to sharing findings related to COVID-19, serum iron binding capacity, serum,... You do not need to reset your password the next time you login under the terms of and... ], and their results are conflicting 39.06 % and specificity of ’. Rahim and Keikhaei examined the diagnostic value order of the subjects in the β-TT group were 10.23 ± (. A test makes it on the contrary, RDW completely failed to differentiate between initiation! Soedirman, Purwokerto, https: //doi.org/10.1088/1755-1315/255/1/012004 in HbA2 levels in patients with hemoglobin ( Hb ) of...

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